Experiment-Strawberries DNA extraction.

Experiment-Strawberries DNA extraction.
There is plenty of video on youtube with that.
Extract DNA at home from a soft fruit using the DNA Extraction protocol linked below. You may find the directions in the View/Print icon on this page. After completing this experiment; reflect on your experience with the DNA extraction. Did you enjoy this experiment? Do you think it was a good learning experience?

DNA Extraction Protocol-Eperiment
This procedure will allow you to extract large amounts of DNA from soft fruits using common household materials.

Materials:

• Fruit to extract DNA from (3-4 strawberries, or 2 cherry tomatoes, or 1/2 full tomato)
• ½ cup water
• 1 tablespoon liquid soap (dish soap, hand soap, or shampoo)
• Pinch of table salt
• Ice cold rubbing alcohol (70-95% isopropyl or ethyl alcohol, left in freezer overnight)
• Small sealable plastic bag
• Small clear glass or plastic container
• Coffee filter or small mesh strainer
• Small cup or bowl

Procedure:
1. Put fruit into small plastic bag with seal (e.g. sandwich bag)
2. Seal the small plastic bag and smash the fruit. Smash the fruit by hand and then roll a pen or marker back and forth over the bag to make the fruit as liquid as possible
3. Add ½ cup water, 1 tablespoon liquid soap, and pinch of salt to the bag
4. Roll the fruit tissue in the liquid for 2 minutes
5. Place coffee filter or strainer on top of a cup or small bowl
6. Pour contents of bag into filter/strainer
7. Set aside for 10 minutes
8. Discard the coffee filter and contents
9. After 10 minutes pour contents of cup/bowl into clear container until the container is 1/3 full
10. Tilt your container and slowly pour rubbing alcohol (70-95% isopropyl or ethyl alcohol) into the container down the side so that it forms a layer on top of the fruit mixture.
11. Pour until you have about the same amount of alcohol in the container as fruit mixture.
12. Wait for the DNA to start precipitating out in the alcohol (the process begins almost immediately and the DNA will continue to condense for the next few minutes)

Alcohol is less dense than water, so it floats on top. Look for clumps of white stringy stuff where the water and alcohol layers meet.

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